MersV1, Main, Exploration, bibRecord, 004021

Synthesis and evaluation of oligodeoxynucleotides containing acyclic nucleosides: introduction of three novel analogues and a summary.

Identifieur interne : 004021 ( Main/Exploration ); précédent : 004020; suivant : 004022

Synthesis and evaluation of oligodeoxynucleotides containing acyclic nucleosides: introduction of three novel analogues and a summary.

Auteurs : P. Nielsen [Danemark] ; L H Drei E ; J. Wengel

Source :

Bioorganic & medicinal chemistry [ 0968-0896 ] ; 1995.

RBID : pubmed:8612043

Descripteurs français

KwdFr :
- Données de séquences moléculaires, Dénaturation d'acide nucléique, Exonucleases (métabolisme), Hybridation d'acides nucléiques, Nucléosides (synthèse chimique), Oligodésoxyribonucléotides (), Oligodésoxyribonucléotides (métabolisme), Oligodésoxyribonucléotides (synthèse chimique), Structure moléculaire, Séquence nucléotidique.
MESH :
- métabolisme : Exonucleases, Oligodésoxyribonucléotides.
- synthèse chimique : Nucléosides, Oligodésoxyribonucléotides.
- Données de séquences moléculaires, Dénaturation d'acide nucléique, Hybridation d'acides nucléiques, Oligodésoxyribonucléotides, Structure moléculaire, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence, Exonucleases (metabolism), Molecular Sequence Data, Molecular Structure, Nucleic Acid Denaturation, Nucleic Acid Hybridization, Nucleosides (chemical synthesis), Oligodeoxyribonucleotides (chemical synthesis), Oligodeoxyribonucleotides (chemistry), Oligodeoxyribonucleotides (metabolism).
MESH :
- chemical , chemical synthesis : Nucleosides, Oligodeoxyribonucleotides.
- chemical , chemistry : Oligodeoxyribonucleotides.
- chemical , metabolism : Exonucleases, Oligodeoxyribonucleotides.
- Base Sequence, Molecular Sequence Data, Molecular Structure, Nucleic Acid Denaturation, Nucleic Acid Hybridization.

Abstract

Novel flexible oligodeoxynucleotides containing (S)-1-(2,3-dihydroxypropyl)thymine or 2',3'-seco-thymidine nucleoside analogues were synthesized on an automated DNA-synthesizer. Oligodeoxynucleotides with one, two or three acyclic nucleosides incorporated in the middle or in the ends of 17-mers have been evaluated. 3'-End-modified oligomers were significantly stabilized towards 3'-exonucleolytic degradation compared to unmodified analogues and showed acceptable hybridization properties as measured by UV experiments. For oligodeoxynucleotide analogues containing the three novel acyclic monomers in the middle, a more pronounced reduction in duplex stability was observed. All oligodeoxynucleotides containing acyclic nucleoside analogues made so far are evaluated with respect to stability towards 3'-exonucleolytic degradation and hybridization properties.

DOI: 10.1016/0968-0896(94)00143-q
PubMed: 8612043

Affiliations:

Danemark

Le document en format XML

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<term>Nucleic Acid Hybridization</term>
<term>Nucleosides (chemical synthesis)</term>
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<term>Oligodésoxyribonucléotides (métabolisme)</term>
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<term>Structure moléculaire</term>
<term>Séquence nucléotidique</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Exonucleases</term>
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<front><div type="abstract" xml:lang="en">Novel flexible oligodeoxynucleotides containing (S)-1-(2,3-dihydroxypropyl)thymine or 2',3'-seco-thymidine nucleoside analogues were synthesized on an automated DNA-synthesizer. Oligodeoxynucleotides with one, two or three acyclic nucleosides incorporated in the middle or in the ends of 17-mers have been evaluated. 3'-End-modified oligomers were significantly stabilized towards 3'-exonucleolytic degradation compared to unmodified analogues and showed acceptable hybridization properties as measured by UV experiments. For oligodeoxynucleotide analogues containing the three novel acyclic monomers in the middle, a more pronounced reduction in duplex stability was observed. All oligodeoxynucleotides containing acyclic nucleoside analogues made so far are evaluated with respect to stability towards 3'-exonucleolytic degradation and hybridization properties.</div>
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Synthesis and evaluation of oligodeoxynucleotides containing acyclic nucleosides: introduction of three novel analogues and a summary.

Synthesis and evaluation of oligodeoxynucleotides containing acyclic nucleosides: introduction of three novel analogues and a summary.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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